Calcitonin Acetate, Salmon

Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, paget's disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity.\n\nCalcitonin Acetate (Salmon) is a synthetic polypeptide of 32 amino acids in the same linear sequence that is found in clacitonin of salmon origin. MW: 432.\n\nSequence:\nCys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Gly-Thr-Pro-NH2\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Calcitonin.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC.\n\nReconstitution:\nSoluble in water and most aqueous buffers below and above the isoelectric point.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0115-30A

Size

1mg

Form

Supplied as a lyophilized powder. No additives.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Analysis of salmon calcitonin I in the ultimobranchial gland and gill filaments during development of rainbow trout, Oncorhynchus mykiss, by in situ hybridization and immunohistochemical staining. Hidaka Y, Tanaka S, Zoolog Sci 2004 Jun;21(6):629-37 2. Overex pression, purification and characterization of recombinant salmon calcitonin, a therapeutic protein, in Streptomyces avermitilis. Chakraborty C, Nandi S, Protein Pept Lett 2004 Apr;11(2):165-73 3. [Quality of life of patients suffering from osteoporosis treated with alendronate and salmon calcitonin] Sapula RA, Ostrowski T, Wiad Lek 2002;55 Suppl 1:444-7 4. Cytotoxicity evaluation of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. Shah RB, Palamakula A, J Pharm Sci 2004 Apr;93(4):1070-82 5. Physical stability of salmon calcitonin spray-dried powders for inhalation. Chan HK, Clark AR, Kuo MC, Vehring R, J Pharm Sci 2004 Mar;93(3):792-804 6. Amylin-induced suppression of ANP secretion through receptors for CGRP1 and salmon calcitonin. Piao FL, Cao C, Kim SZ, Regul Pept 2004 Mar 15;117(3):159-66.