Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilution:
Immunohistochemistry (frozen): 1:1,000-1:3,000
Immunohistochemistry (paraffin): 1:1,000-1:3,000
Immunofluorescence: 1:10-1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.