Calcitonin Gene Related Peptide, Human, Control Peptide (CGRP)

Control Peptide for C0115-02D\n(antiserum) \n\nA 19aa Peptide mapping at the N-terminus of human CGRP .\nSpecies sequence homology: Equine, sheep, porcine, canine, rat, bovine, frog, monkey, zebra fish (84-95%).\n\nCalcitonin leads to a lowering of plasma calcium and phosphate levels via the inhibition of bone resorption and excretion through the kidneys. Calcitonin is found in C cells of the normal and hyperplastic thyroid. \n\nThe calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37-aa peptide produced by tissue specific processing of the calcitonin gene. \n\nCalcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37-aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52-aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney. ADM has specific receptors on astrocytes and it is unregulated in ischaemia. \n\nThe calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking, Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nAntibody Blocking: \n5-10ug control peptide per 1ug of IgG or 1ul of antiserum\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0115-02P

Size

100ug

Applications

ELISA

Reactivities

Hum, Rat, Bov, Can, NHP, Prc, Sheep

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% sodium azide. Reconstitute with 100ul sterile PBS.

Purity

Highly purified

References

1. Steenbergh et al (1986) FEBS Lett. 209, 97-103; 2. Petermann JB et al (1987) JBC 262, 542-545; 3. Wimalwamsa SJ et al (1990) BBRC 167, 993-1000; 4. Kitamura k et al (1992) BBRC 185, 134-141; 5. McLatchie LM et al (1998) Nature 393, 333-339; 6. Nagae T et al (2000) BBRC 270, 89-93; 7. Husmann, K et al (2000); Mol Cell Endocrinol (2000) 162, 35-43