Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternate splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%-72% aa sequence identity with mouse and rat CACLR.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.25ug/ml. Validated for flow cytometry using MCF-7 cells. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.
MCF-7 cells were stained with anti-CALCR (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.
Hybridoma:
Mouse myeloma with B cells obtained from a mouse immunized with HEK293 cells transfected with human CALCR (Accession # P79222).
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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