Calcitonin Receptor Like Receptor, Rat Control Peptide (CRLR)

Control Peptide for C0115-90 (antiserum) and C0115-85 (affinity purified)\n\nThe calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175aa; rat 182aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nAntibody Blocking: 5-10ug per 1ul C0115-90 (antiserum) or per 1ug C0115-85 (affinity purified antibody).\nELISA: 50-100ng/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0115-80

Size

50ug

Applications

ELISA

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. Njuki, et al., Clin. Sci. 85: 385-388 (1993). 2. Aiyar, N., et al., J. Biol. Chem. 271: 11325-11329 (1996). 3. Fluhmann, B., et al., BBRC 206: 341-347 (1995). 4. McLatchie, L.M., et al., Nature 393: 333-339 (1998). 5. Nagae, T., et al., BBRC 270 89-93 (2000). 6. Chakravarty, P., et al., B.r J. Pharmacol. 130: 189-195 (2000). 7. Aldecoa, A., et al., FEBS Lett. 471: 156-160 (2000). 8. Husmann, K., et al., Mol. Cell. Endocrinol. 162: 35-43 (2000).

Additional Info

Recognizes rat Calcitonin Receptor Like Receptor. No significant sequence homology is detected with other CRLR. Species Sequence Homology: Mouse: 90%; rhuman and chimp: 70%; porcine: 64%.