The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. (NCBI Entrez Gene Summary).
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-2ug
Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.