Calgranulin A,B (MRP8 and MRP14) are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S100 protein family. Peripheral blood monocytes carry the antigen extra and intracellularly, neutrophils only intracellularly. The antigen is present on macrophages in the red pulp of the spleen and in the liver. It is also strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia). Calgranulin is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis, some GVDH and squamous cell carcinoma.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:8000, 0.3-1ug/ml
Western Blot: 0.3-1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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