Calmodulin (CALM 1, CALM 2, CALM 3, CALM, CALM1, CALM2, CALM3, CALML2, CAM 1, CAM 2, CAM 3, CAM I, CAM1, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CaM, Calmodulin 1, Calmodulin1, DD132, PHKD, Phosphorylase kinase delta, Phosphorylase kinase delta subunit)

Cat no: C1035-01C

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17kD protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: Detects a 17kD protein representing calmodulin from Dictyostelium cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C1035-01C
Reactivities:	Rat, Bovine, Chicken/Bird
Hosts:	Mouse
Applications:	ELISA, Immunocytochemistry, Western Blot
Size:	200ul
Form:	Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
P type:	Mab
Isotype:	IgG1
Purity:	Ascites
References:	This product has been used in:Hulen D et al. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil Cytoskeleton 18:113-22 (1991). Zhu Q et al. Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum. J Cell Sci 104 (Pt 4):1119-27 (1993). Zhu Q & Clarke M Association of calmodulin and an unconventional myosin with the contractile vacuole complex of Dictyostelium discoideum. J Cell Biol 118:347-58 (1992). Coling DE & Salisbury JL Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae). J Protozool 39:385-91 (1992). Clarke M et al. Density-dependent induction of discoidin-I synthesis in exponentially growing cells of Dictyostelium discoideum. Differentiation 34:79-87 (1987).
Additional info:	This antibody detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Species Crossreactivity: Crossreacts with bovine, chicken, E. coli, rat, Chlamydomonas reinhardtii and Dictyostelium discoideum.

See Full Product Listings >

Calmodulin (CALM 1, CALM 2, CALM 3, CALM, CALM1, CALM2, CALM3, CALML2, CAM 1, CAM 2, CAM 3, CAM I, CAM1, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CaM, Calmodulin 1, Calmodulin1, DD132, PHKD, Phosphorylase kinase delta, Phosphorylase kinase delta subunit)

Cat no: C1035-01C

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*