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Calmodulin-Dependent Protein Kinase II beta (CAMKIIb, MGC29528)

Calmodulin-Dependent Protein Kinase II beta (CAMKIIb, MGC29528)

Cat no: C1035-25Q4

Supplier: United States Biological
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The multifunctional Ca2+/ calmodulin dependent protein kinase II also known as Calcium/calmodulin Kinase (CaMK II) is a well known effector of calcium and calmodulin-mediated functions. It is present in many tissue but it is abundant in brain. CaMKII also required for synaptic plasticity such as Long Term Potentiation (LTP), a cellular model for learning and memory. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. Immunocytochemistry: 1:100. Immunofluorescence: 1:100. (Staining can be weak.) Optimal dilutions to be determined by the researcher. Positive Controls: Human, mouse or rat brain Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C1035-25Q4
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Rochlitz,H., Voigt,A., et al. Cloning and quantitative determination of the human Ca2+/calmodulin-dependent protein kinase II (CaMK II) isoforms in human beta cells Diabetologia 43, 465-473 (2000) 2. Tombes,R.M. and Krystal,G.W. Identification of novel human tumor cell-specific CaMK-II variants Biochim. Biophys. Acta 1355, 281-292 (1997).
Additional info: Recognizes human CAMKIIb at ~68kD. Species Crossreactivity: mouse and rat.

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