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Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2)

Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2)

Cat no: C1035-25G2

Supplier: United States Biological
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CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1035-25G2
Reactivities: Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5,150mM sodium chloride,100ug/ml BSA, 50% glycerol.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes rat CAMK2 when Phosphorylated at Thr286. Species sequence homology: Xenopus, human and mouse.

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