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Calnexin (CANX, CNX, FLJ26570, Histocompatibility Complex Class I Antigen Binding Protein p88, IP90, P90) (Azide Free)

Calnexin (CANX, CNX, FLJ26570, Histocompatibility Complex Class I Antigen Binding Protein p88, IP90, P90) (Azide Free)

Cat no: C1035-26J3

Supplier: United States Biological
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Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1035-26J3
Reactivities: Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Guinea Pig, Non-Human Primate, Porcine, Sheep, Xenopus/Amphibian
Hosts: Rabbit
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 50ul
Form: Supplied as a liquid.
P type: Pab
Isotype: IgG
Purity: Serum.
References: 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
Additional info: Recognizes canine calnexin. Species Crossreactivity: human, mouse, rat, bovine, chicken, guinea pig, hamster, porcine, monkey, sheep, xenopus.

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