Home  >  Products  >  Calnexin - ER Marker
Calnexin - ER Marker

Calnexin - ER Marker

Cat no: C1035-26G

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry/Immunofluorescence: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100, using antigen retrieval with sodium citrate buffer (pH 6.0). Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells, HeLa whole cell lysate, Mouse bladder tissue. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1035-26G
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid (serum) in PBS, containing 50% glycerol aand sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
Alternative names: CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90
References: Hammond C & Helenius A Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126:41-52 (1994). Ou WJ et al. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364:771-6 (1993). Wada I et al. SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem 266:19599-610 (1991).
Additional info: Recognizes human Calnexin at ~90 kD (actual weight: 97kD). Species Crossreactivity: mouse. Cellular Localization: Endoplasmic reticulum.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave