Calpain, mu

This protein consists of an 80kD large subunit and a 30kD small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.\n\nApplications: \nThis protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.\n\nFor adsorption we suggest the following procedure: A- Dilute 1microl of the antiserum against micro-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1microg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).\n\nAs a result, the immunostaining should be strongly reduced or even completely prevented.\n\nStability: \nm-Calpain although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C1035-33M

Size

2ug

Form

Supplied as a liquid in 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.

Purity

(same/more than) 90% as determined by reducing and non-reducing SDS-PAGE Coomassie.

References

1. Leupeptin, a calpain inhibitor, protects inner ear hair cells from aminoglycoside ototoxicity.\n\nTohoku J Exp Med 2006 Jun;209(2):89-97\n\n2. Differential regulation of platelet aggregation and aminophospholipid exposure by calpain.\n\nBr J Haematol 2006 May;133(4):419-26\n\n3. Interaction between catalytically inactive calpain and calpastatin. Evidence for its occurrence in stimulated cells.\n\nFEBS J 2006 Apr;273(8):1660-8\n\n4. Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions.\n\nCancer Res 2006 Apr 15;66(8):4273-8\n\n5. Mu-calpain is involved in the regulation of TNF-alpha-induced matrix metalloproteinase-3 release in a rheumatoid synovial cell line.\n\nBiochem Biophys Res Commun 2006 May 12;343(3):937-42\n\n6. Early onset calpainopathy with normal non-functional calpain 3 level.\n\nDev Med Child Neurol 2006 Apr;48(4):304-6