Calsequestrin-2 (CASQ2, CSQ, Calsequestrin cardiac muscle isoform)

Calsequestrin is the major calcium storage protein of the sarcoplasmic reticulum. Intraluminar Ca2+ binds to calsequestrin during diastole to prevent Ca2+ precipitation and to lower its free ionic concentration to facilitate efficient storage. During systole, Calsequestrin coordinately releases ~40-50 Ca2+ ions per molecule for each contraction-relaxation cycle by an uncertain mechanism. Calsequestrin has been shown to be of major importance in the regulation of cardiac excitation-contraction coupling.\n\nApplications: \nSuitable for use in Western Blot, Absorption and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C1036-05G

Size

2ug

Form

Supplied as a lyophilized powder from 10mM Tris-HCl, 1mM EGTA. Reconstitute in sterile dH20 to (same/more than)0.1mg/ml.

Purity

(same/more than)90% by SDS-PAGE

References

1. Antibodies targeting the calcium binding skeletal muscle protein calsequestrin are specific markers of ophthalmopathy and sensitive indicators of ocular myopathy in patients with Graves' disease. Clin Exp Immunol 2006 Jul;145(1):56-62. 2. SERCA1 and calsequestrin storage myopathy: a new surplus protein myopathy. Brain 2006 May 19. 3. THE CONFORMATION OF CALSEQUESTRIN DETERMINES ITS ABILITY TO REGULATE SKELETAL RYANODINE RECEPTORS. Biophys J 2006 May 12. 4. Gene Expression of the Na-Ca(2+) Exchanger, SERCA2a and Calsequestrin after Myocardial Ischemia in the Neonatal Rabbit Heart. Biol Neonate 2006 Apr 25;90(3):174-184. 5. Abnormal interactions of calsequestrin with the ryanodine receptor calcium release channel complex linked to exercise-induced sudden cardiac death. Circ Res 2006 May 12;98(9):1151-8. 6. Knocking down type 2 but not type 1 calsequestrin reduces calcium sequestration and release in C2C12 skeletal muscle myotubes. J Biol Chem 2006 Jun 2;281(22):15572-81.