CaMKII, one of the most abundant neuronal protein kinases was first identified in neuronal membrane fractions. CaMKII phosphorylates a broad range of substrates and is expected to have pleiotropic regulatory actions in cellular differentiation and mature neuronal functions. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha subunit of CaMKII is identified as the major postsynaptic density protein (mPSDp). It undergoes calcium/calmodulin-dependent autophosphorylation on Thr286 in its regulatory domain, rendering the kinase partially calcium-independent. Thus this Thr286 autophosphorylation controls subcellular targeting of CaMKII in neurons with important functional consequences. The protein is enriched in brain and its overexpression alters the general cell growth, differentiation, and morphology of neurons. human CaMKII gene maps on chromosome 5 at 5q33.1.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
Mouse brain tissue
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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