CaM Kinase IV (Calmodulin-Dependent Protein Kinase IV, CAMKIV)

Ca2+/calmodulin dependent protein kinase type 4 (CaMK4, formerly known as CaM kinase Gr) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase. CaMK4 is highly expressed in cell and tissue extracts such as the brain, lymphocytes, spleen, neurons, thymus and testes. This kinase has two isoforms, a catalytically active calmodulin dependent kinase or a testes specific calmodulin binding protein, calspermin, generated by alternative splicing from the same gene. CaMK4 appears to require another kinase (CaMKK) to stimulate it. CaMK4, in a calcium and calmodulin dependent manner, is implicated in phosphorylating transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium dependent gene expression. In neurons, CaMK4 is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T cells (NFAT). CaMK4 is encoded, along with calspermin, by the CaMK4 gene. It has been found that in testes, CaMK4 is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.