Mouse CDO is a 190kD member of the Ig/FN-type III repeat subfamily of transmembrane proteins. The extracellular region contains five Ig-like domains and three FN-III modules. The molecule forms cis-complexes with itself and the related molecule termed BOC. Mouse CDO extracellular domain shares 95% and 85% aa identity with the corresponding regions of rat and human CDO, respectively.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml with the appropriate secondary reagents to detect mouse CDO. Using a colorimetric detection system, the detection limit for rmCDO is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
ELISA (direct): 0.5-1.0ug/ml with the appropriate secondary reagents to detect mCDO. The detection limit for rmCDO is approximately 1ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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