Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activate calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry (Paraffin). Other applications not tested.
Recommended Dilution:
Western Blot: 1:5000-10000
Immunohistochemistry: 1:100-250 (Paraffin)
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.
Positive Control:
Human brain, mouse brain, rat brain, and SHSY5Y lysates.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.