The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which
contain a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it
exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52000.
Applications:
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Horseradish peroxidase/IgG protein molar ratio (E/P): ~1.3
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.