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Canavalia ensiformis (Concanavalin A, Jackbean) (HRP)

Cat no: C1045-08A


Supplier: United States Biological
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The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52000. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Horseradish peroxidase/IgG protein molar ratio (E/P): ~1.3 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C1045-08A
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Purified by DEAE chromatography.
Additional info: Recognizes Canavalia ensiformis. Gives one characteristic precipitin line when tested in Immunoelectrophoresis.

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