Canine adrencocorticotropic hormone ELISA Kit

This kit is used for the quantitative determination the concentrations of Canine Adrenocorticotropic Hormone (ACTH) in canine serum, plasma, cell culture supernatants, and other biological fluids. ##Important notes (read before start)## 1. The kit should be balanced 15-30 minutes in the room temperature after taken out from the refrigeration environment. The microplate should be stored in ##sealed bag and valid for 30 days after open##. 2. Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from contamination. 3. Washing buffer may have crystallized precipitates, heating the solution can dissolve the precipitates. 4. Add samples as soon as possible. Adding solutions by multi-channel pipettes is highly recommended. 5. Dilute the samples before the assay by the same buffer which has been used to extract the samples, if the concentration of original samples is higher than the assay range. 6. Duplicated loading of all standards and samples is highly recommended. 7. If OD450 in both standard and samples are very low, the incubation time for each step can be extended. 8. The substrates are light sensitive; avoid any unnecessary exposure of the substrate under the light. Avoid any contact of the substrates with metal which prevents color development. 9. Due to the possibility of mismatching between antigen from other resource and antibody used in our kits (e.g. antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins/molecules may not be recognized by this kit. 10. Influenced by the factors including cell viability, cell numbers, samples stored for long time, or samples from cell culture supernatant without concentrated, these samples may not be detected by this kit. 11. Samples may be stored at 2-8°C if test happens within one week after collection. Otherwise, samples must be stored at -20°C for less than two months or -80°C for less than six months. 12. Customer is responsible for the sample consumed during the assay. Customer should calculate the possible amount of the samples used in the entire assay and reserve sufficient samples in case of any testing issue. 13. Do not mix or interchange reagents between different lots. 14. Do not use reagents beyond the expiration date of the kit.