CANT1, Recombinant, Human (Putative NF-kappa-B-activating Protein 107, Putative MAPK-activating Protein PM09, Apyrase Homolog, Soluble Calcium-activated Nucleotidase 1, SCAN-1, SHAPY)

Calcium Activated Nucleotidase 1 (CANT1), is a Ca2+-dependent nucleoside diphosphatase which can hydrolyze a variety of nucleoside di- and triphosphates. CANT1 is also known as SCAN-1 and SHAPY. Its preferred substrates are UDP and GDP, and it is not active against nucleoside monophosphates (1). The CANT1 gene encodes a Type II membrane protein that is anchored in the endoplasmic reticulum (ER) membrane with its catalytic domain in the ER lumen (2). A secreted, soluble form of the enzyme also exists (1). CANT1 is expressed in many tissues, including fibroblasts and chondrocytes. Mutations of CANT1 are a cause of Desbuquois dysplasia, a condition characterized by deformation of bones and joints (2, 3). Recombinant human CANT1 was expressed as a secreted, soluble protein.\n\nSource:\nRecombinant corresponding to aa80-401 from human CANT1, fused with 6-His tag at N-terminal, expressed in NS0 cells.\n\nMolecular Weight: \n~37kD\n\nBiological Activity:\nMeasured by its ability to hydrolyze the B5'-phosphate group from the substrate uridine-5'-diphosphate (UDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >85,000 pmol/min/ug, as measured under the described.\n\nEndotoxin Level:\n(same/less than)1EU/1ug (LAL)\n\nStorage and Stability::\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C1075-78C

Size

10ug

Form

Supplied as a liquid in Tris, sodium chloride, glycerol.

Purity

~95% (SDS-PAGE)

References

1. Smith, T. et al. (2002) Arch. Biochim. Biophys. 406:105. 2. Failer, B.U. et al. (2002) J. Biol. Chem. 277:36978. 3. Huber, C. et al. (2009) Am. J. Hum. Genet. 85:706. 4. Faden, M. et al. (2010) Am. J. Med. Genet. A. 152A:1157.