Home  >  Products  >  Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, ALYE870, FLJ22167, GlcNAc6ST-3, GST4-alpha, hIGn6ST, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, MGC74625, PRO1886)
Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, ALYE870, FLJ22167, GlcNAc6ST-3, GST4-alpha, hIGn6ST, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, MGC74625, PRO1886)

Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, ALYE870, FLJ22167, GlcNAc6ST-3, GST4-alpha, hIGn6ST, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, MGC74625, PRO1886)

Cat no: C1103-05

Supplier: United States Biological
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CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc. Human CHST5 is a 390aa type II transmembrane protein. It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358). There is one alternate start site that is 22aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa28-390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4- beta. Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C1103-05
Reactivities: Human
Hosts: Goat
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282. 2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849. 3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447. 4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899. 5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979. 6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964. 7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613. 8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
Additional info: Recognizes human CHST5.

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