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Carbonic Anhydrase 1, Recombinant Human, aa1-261, His-Tag (CA1)

Cat no: C1105-44

Carbonic Anhydrase 1, Recombinant Human, aa1-261, His-Tag (CA1)

Carbonic anhydrase 1(CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O ? HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.\n\nRecombinant protein corresponding to human Carbonic anhydrase1, his-tagged, expressed in E. coli (NP_001122301). \n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

C1105-44

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.NOgr

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