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Carbonic Anhydrase VI (Salivary Carbonic Anhydrase, Gustin)

Carbonic Anhydrase VI (Salivary Carbonic Anhydrase, Gustin)

Cat no: C1105-65A

Supplier: United States Biological
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Carbonic Anhydrase VI (CA6), also known as gustin and salivary carbonic anhydrase, is a secreted zinc metalloenzyme that is a major protein component of parotid saliva and milk. It carries out a mucosal protective function by regulating pH balance in the upper alimentary and respiratory tracts. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Intracellular Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-5x10e5 OVCAR-3 cells in a total reaction volume (same/less than) 200ul. For intracellular staining, cells must first be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C1105-65A
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human Carbonic Anhydrase VI (CA6). Does not crossreact with recombinant human CA1, -2, -3, -4, -5A, -5B, -7, -8, or -9. Species Sequence Homology: Bovine (66%), mouse (50%).

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