Carbonyl reductase 3 (CBR3) is members of the family of short chain dehydrogenases/reductases. Carbonyl reductases are two monomeric carbonyl reductases, carbonyl CBR1 and CBR3. CBR3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase.
Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000-1:2000
Western Blotting: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.