Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase (PIK3). Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated on Thr308 in the catalytic domain and Ser473 in the C-terminal regulatory domain. Several protein partners for PKB have been identified. CTMP, a carboxyl terminal modulator protein, binds to PKB-a and negatively regulates PKB activity by inhibiting phosphorylation of these PKB residues. CTMP (mouse 248aa, human 240aa, chromosome 1q21, 22-26kD) predominantly expressed in skeletal muscle, testis, uterus, brain, and kidney, with lower levels in heart, liver, and lung. The presence of multiple transcripts suggested that CTMP undergoes alternative splicing.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (Formalin-fixed paraffin sections): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
