CPA1 is an exopeptidase mainly produced by the pancreas. It cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. CPA1 is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The amino acid sequence of human CPA1 is 88%, 82%, and 77% identical to that of dog, rat/mouse, and chicken, respectively.
Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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