Carboxypeptidase B, PMSF Treated

Carboxypeptidase B (CPB) catalyzes hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-teminal position in polypeptides. It occurs as a zymogen in the pancreas of most vertebrates.\nSource:\nPorcine pancreas\nPurity:\nChromatographically purified \nForm:\nSupplied as a solution in 0.1M sodium chloride. Treated with PMSF to inhibit protease activity.\nActivity:\n(same/more than) 70 units/mg protein\n(same/more than) 7000 units/ml\nChymotrypsin (%):\n0.003\nTrypsin (%):\n0.002\nCarboxypeptidase (%):\n0.32\nUnit Definition:\n1 unit hydrolyzes 1 micromole of hippuryl-L-arginine per minute at 25 degrees C, pH 7.65.\nStorage and Stability:\nThe untreated enzyme is stable for 9-12 months when stored at -20C. \nQuality Control:\nSDS-PAGE

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SPECIFICATIONS

Catalog Number

C1205-05

Size

1000U

References

Folk, J. E., Piez, K. A., Carroll, W. R. and Gladner, J., J. Biol. Chem., 235:2272 (1960).\nAkanuma, H., Kasuga, A., Akanuma, T., and Yamasaki, M.: The Effective Use of Affinity Chromatography for the Study of Complex Formation of Bovine Carboxypeptidase B with Basic and Aromatic Acid Analogues, Biochem Biophys Res Commun 45, 27, 1971\n

Alternative Names

EC=3.4.17.2; Porcine pancreas; Protaminase; Peptidyl-L-Lysine [L-Arginine] hydrolase