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Carcinoembryonic Antigen (CEA)

Carcinoembryonic Antigen (CEA)

Cat no: C1299-87O

Supplier: United States Biological
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The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. In the past, clinical utility of CEA antibodies in immunopathology has been limited by cross-reactivity with CEA-related antigens, particularly Non-Specific Cross-Reacting Antigen (NCA), in normal colon mucosa and human granulocytes. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry Staining: Brightness 80, Cytoplasmic staining 3+, Membrane staining 2+, Background 2+, Staining of mucus 2+. Optimal dilutions to be determined by the researcher. Recommended antibody pair for sandwich immunoassay: Capture: C1299-87W Detection: C1299-87O Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1299-87O
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry
Size: 1mg
Form: Supplied as a liquid in 10mM Tris, pH7.5, 0.15M sodium chloride, 0.05% sodium azide. Also available without azide. See C1299-87S.
P type: Mab
Isotype: IgG1
Purity: ~90% by SDS-PAGE. Purified by ion-exchange chromatography.
Additional info: Recognizes human carcinomas. Epitope specificity group IVa or II. Does not crossreact with human leukocytes in cytofluorometry.

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