CEA can be found in a large variety of carcinomas, but also in the tissue of non-neoplastic diseases and normal epithelia. Despite this lack of specificity, immunohistochemical detection of CEA is frequently used for the histopathologial diagnosis of human tumors.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Frozen, paraffin): 1:5-1:10 using the Indirect method. Fix frozen sections with acetone for 5 min. at -20C. Pretreatment not required for paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive Control:
Colonic carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.