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CARD10 (Caspase Recruitment Domain-containing Protein 10, CARD-containing MAGUK Protein 3, Carma 3, CARMA3)

CARD10 (Caspase Recruitment Domain-containing Protein 10, CARD-containing MAGUK Protein 3, Carma 3, CARMA3)

Cat no: C1350-10G

Supplier: United States Biological
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CARMA proteins are scaffold molecules that belong to the membrane-associated guanylate kinase-like (MAGUK) and the caspase-associated recruitment domain (CARD) protein families. They play critical role in antigen receptor signaling pathways. CARMA1, CARMA2, and CARMA3 share homologous sequence and function but are found in different tissues. CARMA1 is predominantly located in lymphoid tissue. CARMA1 protein associates with lipid rafts and interacts with Bcl10, that is required for activation of TCR induced NFkB signaling pathway. Specifically, CARMA1 N-terminal CARD domain binds to Bcl10 CARD motif. It is also essential for protein- induced IL-2 and INF-g production. CARMA genes deactivation studies in mice resulted in reduced immunoglobulin levels and absence of TCR-induced NFkB activation. This suggests that CARMA mutations may lead to adaptive and innate immunity malfunction. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry: 2.5-20ug/ml (10ug/ml-optimal) Optimal dilutions to be determined by the researcher. Positive Control: NIH/3T3 and Jurkat whole cell lysates at ~130kD, human tissues including heart, kidney, small and large intestine, testies, and skeletal muscle Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1350-10G
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Gaide, O et al. (2002) Nature Immunology 3: 836-843. 2. Hara, H. et al. (2004) JEM, 200(9): 1167-1177. 3. Narayan, P. et al. (2006) Mol Cell Bio 26(6): 2327-2336. 4. Egawa T. et al. (2003) Current Biology 13: 1252-1258. 5. Grabiner, B. et al. (2007) Genes and Dev. 21: 984-996. 6. Newton, K. et al. (2003) Current Biology 13: 1247-1251. 7. Stilo R. et al. (2004) J. Biol. Chem. 279: 34323-34331.
Additional info: Recognizes human CARMA3.

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