Cardiotrophin-1 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Cardiotrophin-1 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nCardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ16619\n\nEntrez Gene ID:\n1489\n\nKit Components:\n96-well plate pre-coated with anti-Cardiotrophin-1, Human\nLyophilized recombinant Cardiotrophin-1, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143738

Size

96Tests

References

1. Pennica, D.; Swanson, T. A.; Shaw, K. J.; Kuang, W.-J.; Gray, C. L.; Beatty, B. G.; Wood, W. I. : Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization. Cytokine 8: 183-189, 1996.