Carnitine Palmitoyl Transferase 1, Liver, Mouse, Control Peptide (CPT1-L)

A 13-aa peptide sequence mapping near the C-terminus of mouse CPT1-L (1).\n\nIn cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.\n\nMitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-1 liver or CPT1A or LCPT-1 (mouse 764-aa, rat 773-aa, human 773-aa, ~88-kda, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial 'contact sites'. It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation. It is predominantly expressed in kidney, liver and in trace amounts in heart. The 'muscle' isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772-aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain. The aa sequences of the two isoforms are ~61% identical.\n\nApplications:\nSuitable for use in ELISA or Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml per well\nAntibody Blocking: 5-10ug\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C1385-31

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide

Purity

Highly purified

References

(1) Cox KB et al (1998) Mamm. Genome 9, 608-610; Uenka R et al (1996) J. Biochem. 119, 533; Britton CH et al (1995) PNAS 92, 1984; Britton CH et al (1997) Genomics 40, 209-211.

Additional Info

Species sequence homology: Rat 84% and human 69%.