Casein Kinase 2 alpha, Recombinant, Human (CKIIa CK2A1, CSNK2A1)

Recombinant Human Casein Kinase 2 alpha is a non-glycosylated polypeptide having a molecular mass of 45.1kD.\n\nUnit Definition:\n1U = 1umol/min at 37C; using the synthetic peptide [RRRDDDSDDD].\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nSpecific activity > 1U/mg\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2085-69

Size

3ug

Form

Supplied as a liquid in 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Casein kinase II alpha subunit and C1-inhibitor are independent predictors of outcome in patients with squamous cell carcinoma of the lung. O-charoenrat P, Rusch V, Sarkaria I, Rao P, Clin Cancer Res 2004 Sep 1;10(17):5792-803 2. Resolution of conflicting assignments for the bovine casein kinase II alpha (CSNK2A2) gene. Aasland M, Vage DI, Klungland H, Anim Genet 2000 Apr;31(2):131-4 3. Isolation, characterization and disruption of the casein kinase II alpha subunit gene of Leishmania chagasi. Bhatia A, Sanyal R, Gedamu L, Mol Biochem Parasitol 1998 May 1;92(2):195-206 4. Theileria-mediated constitutive expression of the casein kinase II-alpha subunit in bovine lymphoblastoid cells. Shayan P, Ahmed JS, Parasitol Res 1997;83(6):526-32 5. Casein kinase II alpha transgene-induced murine lymphoma: relation to theileriosis in ca ttle. Seldin DC, Leder P, Science 1995 Feb 10;267(5199):894-7 6. The Schizosaccharomyces pombe casein kinase II alpha and beta subunits: evolutionary conservation and positive role of the beta subunit. Roussou I, Draetta G, Mol Cell Biol 1994 Jan;14(1):576-86.