Casein Kinase 2 alpha, Recombinant, Zea mays (CKIIa CK2A1, CSNK2A1)

Recombinant Zea Mays Casein Kinase 2 alpha is a non-glycosylated polypeptide having a molecular mass of 39.2kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nUnit Definition:\n1U = 1umol/min at 37C) using the synthetic peptide [RRRDDDSDDD].\n\nBiological Activity:\nSpecific activity > 1U/mg\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2085-69A

Size

3ug

Form

Supplied as a liquid in 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Purification and characterization of recombinant protein kinase CK2 from Zea mays expressed in Escherichia coli. Riera M, Pages M, Guerra B, Protein Expr Purif 2003 May;29(1):24-32 2. The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme. Battistutta R, Sarno S, Marin O, Eur J Biochem 2000 Aug;267(16):5184-90 3. Expression, purification and crystallization of the catalytic subunit of protein kinase CK2 from Zea mays. Guerra B, Niefind K, Schomburg D, Acta Crystallogr D Biol Crystallogr 1998 Jan 1;54 ( Pt 1):143-5 4. Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 A resolution. Niefind K, Guerra B, Issinger OG, EMBO J 1998 May 1;17(9):2451-62.