Casein Kinase 2 beta, Recombinant, Human (CK2b, CK2 beta, (CKIIb CK2B1, CSNK2B1)

Recombinant Human Casein Kinase 2 beta is a non-glycosylated polypeptide having a molecular mass of 24.9kD.\n\nUnit Definition:\n1U = 1umol/min at 37C) using the synthetic peptide [RRRDDDSDDD].\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nSpecific activity > 1U/mg\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2085-72

Size

3ug

Form

Supplied as a liquid in 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Cloning and expression in Escherichia coli of a new gene of Schistosoma japonicum encoding casein kinase II beta subunit. Peng ZY, Yu XB, Xu J, Chin Med J (Engl) 2004 Sep;117(9):1321-5 2. Localization of the casein kinase II beta-subunit gene within the mouse H-2 complex class III region and comparison of expression with Bat genes. Lanning D, Lafuse WP, Mamm Genome 1997 Jul;8(7):519-21 3. The casein kinase II beta subunit binds to Mos and inhibits Mos activity. Chen M, Li D, Cooper JA, Mol Cell Biol 1997 Apr;17(4):1904-12 4. Localization of eight additional genes in the human major histocompatibility complex, including the gene encoding the casein kinase II beta subunit (CSNK2B). Albertella MR, Jones H, Olavesen MG, Genomics 1996 Sep 1;36(2):240-51 5. Localisation of eight additional genes in the human major histocompatibility complex, including the gene encoding the casein kinase II beta subunit, and DNA sequence analysis of the class III region. Albertella MR, Jones H, Olavesen MG, DNA Seq 1996;7(1):9-12 6. The protein kinase from mitotic human cells that phosphorylates Ser-209 on the casein kinase II beta-subunit is p34cdc2. Litchfield DW, Bosc DG, Biochim Biophys Acta 1995 Oct 19;1269(1):69-78.