Caspase 1, Active, Recombinant, Human

Caspase 1 (also know as ICE, Interleukin-1b-Converting Enzyme) is the prototypical member of the caspase family of cysteine proteases. Caspase 1 exists in cells as an inactive 45kD pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kD) and small (10kD) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of Caspase 1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the Caspase 1 is present in the inactive pro-enzyme form. \nThe active Caspase 1 preferentially cleaves Caspase 1 substrates (e.g., YVAD-AFC or YVAD-pNA). The active recombinant Caspase 1 is routinely tested for its ability to enzymatically cleave these two substrates.\n\nRelated Products:\nC2086-67: Caspase 1, Fluorometric Substrate, YVAD-AFC (Ac-Tyr-Val-Ala-Asp-AFC)\n\nC2086-68: Caspase 1 (ICE) Colorimetric Substrate, YVAD-pNA\n\nSpecific Activity:\n~5000 units/mg\n\nUnit Definition:\nOne unit of the recombinant Caspase 1 is the enzyme activity that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37 degrees C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% Chaps, 10mM EDTA, 5% glycerol and 10mM DTT.\n\nApplications:\nSuitable for use in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in Caspase 1 activity assays. Other applications not tested.\n\nRecommended Dilutions:\nAnalyzing Caspase Activity: 1 unit per assay \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -70 degrees C. Stable for 12 months at -70 degrees C. Reconstitute with sterile PBS, 15% glycerol. Aliquot to avoid repeated freezing and thawing. Store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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