Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer and 2X Reaction Buffer, and DTT used for caspase assays are also available separately.
Sequence: Ac-Tyr-Val-Ala-Asp-AFC (AFC, 7-amino-4-trifluoromethyl coumarin)
Purity: (same/more than) 98% by HPLC analysis
Concentration: 4mM in DMSO
Storage & Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for a minimum of 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
