Ready-to-use colorimetric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate YVAD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for large volume users.
Sequence: Ac-Tyr-Val-Ala-Asp-pNA
Purity: (same/more than) 98% by HPLC analysis
Storage & Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for a minimum of 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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