Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase-12 (CASP12) process inflammatory cytokines. It attenuates the macrophage-elicited T helper cell type 1 and type 2 cytokine response, with probable compensatory enabling of T cell-derived interferon-gamma formation. Casp12Dmpened the production of the proinflammatory cytokines interleukin 1-beta, IL-18 and IFNg, but not tumor necrosis factor-alpha and IL-6, in response to various bacterial components that stimulate Toll-like receptor and NOD pathways. In rodents, Casp12 mediates apoptosis in response to endoplasmic reticulum stress. However, in human, CASP12 appears to be nonfunctional. A SNP in CASP12 in humans results in the synthesis of either a truncated protein (Csp12S) or a full-length caspase proenzyme (Csp12L). SNP encoding Csp12L is confined to populations of African descent and confers hyporesponsiveness to lipopolysaccharide-stimulated cytokine production in ex vivo whole blood but has no significant effect on apoptotic sensitivity. PCR analysis detected highest expression of CASP12 in lung. Expression was intermediate in small intestine, stomach, and kidney. Nine CASP12 splice variants are identified in lung and small intestine.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.25-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Skeletal muscle
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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