Ready-to-use colorimetric substrate for caspase-2/Ich-1 and related caspases that recognize the amino acid sequence VDVAD. Caspase-2 and related caspase activity can be quantified by detection of free pNA after cleavage from the peptide substrate VDVAD-pNA at OD405 nm using a spectrophotometer or plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays.
Sequence: VDVAD-pNA
Purity: (same/more than) 98% by HPLC analysis
Storage & Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for a minimum of 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.