Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.
Components: 25 assays 100 assays
Cell Lysis Buffer 25ml 100ml
2X Reaction Buffer 2ml 4 x 2ml
VDVAD-pNA Substrate (4mM) 125ul 500ul
DTT (1M) 100ul 400ul
Dilution Buffer 25ml 100ml
Stability:
Store kit at -20 degrees C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4 degrees C after opening). All reagents are stable for 1 year under proper storage conditions.
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