Caspase 3, active, Recombinant, Human (Caspase-3, CPP-32, Apoptain, Protein Yama, SREBP Cleavage Activity 1, SCA-1, CASP-3, CASP3, Cysteine Protease CPP32)

Caspase-3 (CASP3) is part of the cysteine-aspartic acid protease (caspase) family. Sequential activation of CASP3 enzyme results in the execution-phase of cell apoptosis/programmed cell death.\n\nCASP3 has been called the "henchman that goes around and executes the cell." It exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. When cleaved, it activates caspases 6, 7 and 9.\n\nCASP3 is also involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's & Huntington disease. High levels are found in lymphocytes, indicating that it is an important mediator of apoptosis in the immune system.CSN-mediated deneddylation is regulated by active CASP3.\n\nCASP3 cleavage of RAD51 results in a functional decrease in RAD51 strand exchange activity and inhibition activity increases RAD51 protein levels. Mice overexpressing human CASP3 have increased susceptibility to degenerative insults. Single nucleotide polymorphisms in CASP3 gene is associated with lung cancer.\n\nSource: \nRecombinant corresponding to 258aa from human CASP3, expressed in E.coli, fused to His-Tag at C-terminus.\n\nMolecular Weight:\n~29.7kD\n\nAA Sequence:\nSGISLDNSYK MDYPEMGLCI IINNKNFHKS TGMTSRSGTD VDAANLRETF RNLKYEVRNK NDLTREEIVE LMRDVSKEDH SKRSSFVCVL LSHGEEGIIF GTNGPVDLKK ITNFFRGDRC RSLTGKPKLF IIQACRGTEL DCGIETDSGV DDDMACHKIP VEADFLYAYS TAPGYYSWRN SKDGSWFIQS LCAMLKQYAD KLEFMHILTR VNRKVATEFE SFSFDATFHA KKQIPCIVSM LTKELYFYHH HHHHHHHH\n\nBiological Activity:\nAt a concentration of 0.5mg/ml one unit will cleave 1umoles of substrate AC-DEVD-P Nitroanilide per minute at pH 7.4 @ 25 degrees C.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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