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Caspase 3 Apoptosis Detection, Colorimetric BioAssay(TM) Kit (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 Apoptosis Detection, Colorimetric BioAssay(TM) Kit (CPP-32, Apoptain, Yama, SCA-1)

Cat no: C2087-20

Supplier: United States Biological
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. Kit Components (100 assays): C2087-20A: Cell Lysis Buffer C2087-20B: 2X Reaction Buffer C2087-20C: DEVD-pNA (4mM) C2087-20D: DTT (1M) C2087-20E: Dilution Buffer Storage and Stability: Store kit at -20 degrees C before use. After opening, store C2087-20A, Cell Lysis Buffer, C2087-20B, 2X Reaction Buffer and C2087-20E, Dilution Buffer at 4 degrees C. Stable for 6 months.
Catalogue number: C2087-20
Size: 25Tests
References: US Biological application reference: Ling-Min, L. et al., (2010) Acta Physiologica Sinica, 62; 93-100. 1. Cartee, L., et al. (2002) Mol. Pharmacol. 61:1313-1321. 2. Collin C., et al. (2001) J. Biol. Chem. 276:37602-37611. 3. Datta R., et al. (2000) J. Biol. Chem. 275:31733-31738. 4. Ding, H.F., et al. (2000) J. Biol. Chem. 275:38905-38911. 5. Doostzadeh J., et al. (2000) J. Biol. Chem. 275:25336-25341. 6. Fujii T., et al. (2000) J. Biol. Chem. 275:7574-7582. 7. Gomez-Angelats M., et al. (2000) J. Biol. Chem. 275:19609-19619. 8. Kown, M.H., et al. (2000) Circulation. 102:228-232. 9. Shain, K.H., et al. (2002) J. Immunol. 168:2544-2553.10.Snow, A.L., et al. (2001) J. Immunol. 167:5404-5411. 11. Casciola-Rosen, L.A., et al., J. Exp. Med., 183:1957-1964 (1996) 12. Lazebnik, Y., et al., Nature, 371:346-347 (1994)

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