This Caspase 3 ELISA Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex. = 400nm and Em. = 505nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
Kit Components:
C2087-12A: Cell Lysis Buffer 1x25ml *
C2087-12B: Coating Buffer 1x10ml *
C2087-12C: Antibody, 20x 1x500ul
C2087-12D: Blocking Buffer 1x15ml *
C2087-12E: Incubation Buffer 1x100ml *
C2087-12F: DTT (1M) 1x400ul
C2087-12G: DEVD-AFC Substrate 1x500ul
C2087-12H: Positive Control 1x10units
C2087-12J: Microtiter Plate 1x12x8wells
Storage and Stability:
Store all components at -20 degrees C. After thawing, store Cell Lysis Buffer, Coating Buffer, Blocking Buffer and Incubation Buffer at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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