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Caspase-3 (CASP-3, Apopain, Cysteine Protease CPP32, CPP-32, Protein Yama, SREBP Cleavage Activity 1, SCA-1, CPP32)

Cat no: C2087-04Q

Caspase-3 (CASP-3, Apopain, Cysteine Protease CPP32, CPP-32, Protein Yama, SREBP Cleavage Activity 1, SCA-1, CPP32)

The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-4ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2087-04Q

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.5mg/ml BSA., 0.01% thimerosal, 50% glycerol.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human Caspase-3. Species Crossreactivity: mouse and rat.

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