Home  >  Products  >  Caspase 3 (caspase 3, apoptosis-related cysteine peptidase, apopain,CASP-3, Caspase-3, CPP32, CPP-32, CPP32B, Cysteine Protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Yama Protein)

Caspase 3 (caspase 3, apoptosis-related cysteine peptidase, apopain,CASP-3, Caspase-3, CPP32, CPP-32, CPP32B, Cysteine Protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Yama Protein)

Cat no: C2087-04M


Supplier: United States Biological
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Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32kD) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Immunohistochemistry (paraffin); 1:25-1:50 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2087-04M
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Fernandes-Alnemri, T., et al. (1994). J. Biol. Chem. 269:30761
Additional info: Recognizes human Caspase-3. Species Crossreactivity: rat.

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