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Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)

Cat no: C2087-11

Supplier: United States Biological
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Fluorometric substrate for Caspase-3. N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay(TM) Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. Storage and Stability: Store at -20 degrees C, protected from light. 6 months under proper storage conditions
Catalogue number: C2087-11
Size: 200Tests
Form: Supplied as a liquid in 1mM DMSO.
Purity: (same/more than) 98% (HPLC)
Alternative names: N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin
References: Product Specific: Nowak G. (2002) J Biol Chem 277:43377-43388. Brian S., et al. (2002) Pharmacol & Exp Therapeut. 302:8-17. General: Xiang, J., et al., Proc. Natl. Acad. Sci. USA, 93:14559 (1996) Nicholson, D.W., et al., Nature, 376:37-40 (1995) Lazebnic, Y.A., et al. Nature, 371:346-349 (1994)

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