Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)

Fluorometric substrate for Caspase-3.\nN-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin\n\nActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay(TM) Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. \n\nReady-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.\n\nStorage and Stability:\nStore at -20 degrees C, protected from light.\n6 months under proper storage conditions

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SPECIFICATIONS

Catalog Number

C2087-11

Size

200Tests

Form

Supplied as a liquid in 1mM DMSO.

Purity

(same/more than) 98% (HPLC)

References

Product Specific:\nNowak G. (2002) J Biol Chem 277:43377-43388.\nBrian S., et al. (2002) Pharmacol & Exp Therapeut. 302:8-17.\n\nGeneral:\nXiang, J., et al., Proc. Natl. Acad. Sci. USA, 93:14559 (1996) \nNicholson, D.W., et al., Nature, 376:37-40 (1995) \nLazebnic, Y.A., et al. Nature, 371:346-349 (1994)

Alternative Names

N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin