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Caspase-3, Subunit p17 (Caspase-3, CASP-3, Apopain, Cysteine Protease CPP32, CPP-32, Protein Yama, SREBP Cleavage Activity 1, SCA-1, CASP3)

Caspase-3, Subunit p17 (Caspase-3, CASP-3, Apopain, Cysteine Protease CPP32, CPP-32, Protein Yama, SREBP Cleavage Activity 1, SCA-1, CASP3)

Cat no: C2087-01V

Supplier: United States Biological
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Caspases belong to the family of cysteine proteases that can be further sub-grouped into the initiator-executioner and proinflammatory caspases. So far ten human caspases have been reported in scientific literature: initiator caspase-2, -8, -9, -12; effector caspase-3, -6, -7; and proinflammatory caspase-1, -4, -5. All caspases are synthesized as catalytically inactive zymogens and become functional after cleavage at specific internal Asp residue and have characteristic cleavage signature xxx-xxx-xxx-Asp. Thus, caspase-3 has N-terminal prodomain (1-28 residues), large 17kD and small 12kD subunits. It also contains internal Asp-Asp-Asp sequence that blocks access to its Ile-Glu-Thr-Asp175 proteolysis site. The site is cleaved under acidic conditions by caspase-9 via direct proteolysis, than cleaved parts form heterodimer, and final step in apoptosis is initiated. Elevated levels of procaspase-3 were observed in certain cancer cells. This suggests that activation mechanism of caspase-3 may be important in cancer therapy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2087-01V
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Srinivasula S et al. (1998) The J. of Biol. Chem. 273(17): 10107-10111. 2. Putt K et al. (2006) Nature Chem. Biol. 2(10): 543-550. 3. Roy S et al. (2001) PNAS 98(11): 6132-6137. 4. Denault J-B et al. (2006) Mol. Cell 23: 523-533. 5. Yacobi K et al. (2004) Endocrinology 145(4): 1943-1951. 6. Chai J et al. (2001) Cell 104: 769-780.
Additional info: Recognizes human Caspase 3. Species Crossreactivity: mouse.

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