Caspase 4, Active, Recombinant, Human (ICErelII, TX, Ich-2)

Caspase-4 (also know as ICErelII, TX, Ich-2) is the member of the caspase family of cysteine proteases. Caspase-4 exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. Active recombinant human caspase-4 was expressed in E. coli. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits caracteristic of the native enzyme. The active caspase-4 is routinely tested at Untied States Biological for its ability to enzymatically cleaves the substrate WEHD-AFC or WEHD-pNA.\n\nApplications: Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.\n\nForm: Supplied as a lyophilized powder.\n\nReconstitution: Reconstitute to 1 unit per ml in PBS.\n\nSpecific Activity: ~5000 units/mg\n\nUnit Definition: One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37 degrees C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% Glycerol, 10mM DTT.\n\nRecommended Dilution: 1 unit per assay for analyzing caspase activity.\n\nStorage & Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. For long term storage, store at -70 degrees C for 1 year. To avoid multiple freeze thaw cycles, reconstituted product should be aliquoted and stored at 70 degrees C. Aliquots are stable for 12 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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